ABSTRACT
Objective: To establish a UPLC-MS/MS method for simultaneous determination of 10 components in Panax notoginseng saponins (PNS), and the study the effect of Bletilla striata polysaccharides (BSP) on the pharmacokinetic parameters of PNS. Methods: Rats were divided into PNS group (P group) and PNS-BSP compatibility group (BP group) by ig administration. The plasma concentration of 10 saponins was determined by UPLC-MS/MS. The pharmacokinetic parameters were calculated by DAS software. Results: The determination method corresponded to the biological sample measurement requirements. Compared with P group, the AUC of Rb1 significantly reduced in BP group (P<0.01) and the AUCs of notoginsenoside R1, Rg1, Rf, Rd, CK, Rc, Rh1 were lower but without significant difference; The total AUC of 10 components significantly reduced in BP group (P<0.01); The plasma concentration of Rg1 in BP group was lower compared with that in P group (P<0.05); The tmax of Rg1, Rb1, and Rf significantly delayed (P<0.01); The tmax of notoginsenoside R1 and Rg2 delayed compared with P group (P<0.05). Conclusion: The established UPLC-MS/MS analysis method is suitable for the simultaneous determination of 10 components in PNS in rat plasma; PNS-BSP compatibility can reduce the plasma concentration of PNS and AUC exposure and prolong the tmax. This illustrates that BSP may increase the exposure levels of PNS in the gastrointestinal tract so that increase the effect in the gastrointestinal tract.
ABSTRACT
The preparation of Chinese materia medica (CMM) possesses the characteristic of holistic function involving multi-component, multi-target, and multi-link. This characters make it difficult to optimize the preparation process and construct the quality standards of CMM. Thus process building and quality evaluation system based on correctly identifying the components that closely related to the efficacy is a key scientific issue in the preparation process design and quality control of CMM. In this paper, a more comprehensive analysis on the feasibility of different research methods was carried out, including the spectral-efficiency relationships, pharmacokinetics, serum medicinal chemistry and serum pharmacology of drugs, the effect of integration effect correlation analysis, virtual screening, and comparative analysis of knock-in/knock-out. The purpose of this article is to provide the reference for identifying the bioactive marker group which is used for the CMM preparation process and quality evaluation.
ABSTRACT
To compare the difference of total phenol of magnolia solid dispersion prepared by different methods. Hot melt extrusion, solvent evaporation method, and fusion-cooling method were used to prepare total phenol of Magnolia accessory solid dispersion, Plastone S-630 and HPC. The drug dispersion state in the prepared solid dispersion was evaluated with DSC and X-ray diffraction; FT-IR method was used to analyze the possible connections between drug and accessories. Finally, accelerated stability-in vivo dissolution test was use to compare the stability differences between these three processes. The results of DSC and X-ray diffraction showed that all of the drug in solid dispersion processed by three processes can exist in amorphous form; FT-IR results also could not distinguish the difference between the three processes; accelerated stability-in vivo dissolution test showed the stability of solid dispersion prepared by HPC was better than Plastone S-630, and the same kinds of materials solid dispersion prepared by hot melt extrusion showed a better stability than the other two processes.